منابع مشابه
Spokes coordinate flagella
C ompetition for the cytoplasmic tail of an integrin allows cells to read different matrix densities, say Millon-Frémillon et al. The tail region of β1 integrin is tiny—only 47 amino acids long. That doesn’t leave much room for the binding of cytoplasmic accessory proteins that help control the integrin’s activation state. When talin holds this position, it fl ips open integrin as a fi rst step...
متن کاملCryoelectron tomography of radial spokes in cilia and flagella
Radial spokes (RSs) are ubiquitous components in the 9 + 2 axoneme thought to be mechanochemical transducers involved in local control of dynein-driven microtubule sliding. They are composed of >23 polypeptides, whose interactions and placement must be deciphered to understand RS function. In this paper, we show the detailed three-dimensional (3D) structure of RS in situ in Chlamydomonas reinha...
متن کاملRadial spokes of Chlamydomonas flagella: polypeptide composition and phosphorylation of stalk components
Polypeptides from flagella or axonemes of Chlamydomonas reinhardtii were analyzed by labeling cellular proteins by prolonged growth on 35S-containing media and using one- and two-dimensional electrophoretic techniques which can resolve greater than 170 axonemal components. By this approach, a paralyzed mutant that lacks axonemal radial spokes, pf14, has been shown to lack 17 polypeptides in the...
متن کاملRadial spokes of Chlamydomonas flagella: genetic analysis of assembly and function
In addition to the previously studied pf-14 and pf-1 loci in Chlamydomonas reinhardtii, mutations for another five genes (pf-17, pf-24, pf-25, pf-26, and pf-27) have been identified and characterized as specifically affecting the assembly and function of the flagellar radial spokes. Mutants for each of the newly identified loci show selective alterations for one or more of the 17 polypeptides i...
متن کاملThree-dimensional structure of the radial spokes reveals heterogeneity and interactions with dyneins in Chlamydomonas flagella
Radial spokes (RSs) play an essential role in the regulation of axonemal dynein activity and thus of ciliary and flagellar motility. However, few details are known about the complexes involved. Using cryo-electron tomography and subtomogram averaging, we visualized the three-dimensional structure of the radial spokes in Chlamydomonas flagella in unprecedented detail. Unlike many other species, ...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 2008
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.1802iti3